In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain.
It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.
It can be used to identify the yeast-like fungus Pneumocystis jiroveci,[1] which causes a form of pneumonia called Pneumocystis pneumonia (PCP) or pneumocystosis.
The cell walls of these organisms are outlined by the brown to black stain.
The principle of GMS is the reduction of silver ions, which renders the fungal cell wall black. The fungal cell wall commonly contains polysaccharides. In a GMS procedure, chromic acid is first used to oxidize polysaccharides, generating aldehydes. Then Grocott's alkaline hexamine-silver solution is applied, where the silver ions are reduced to black amorphous silver. The reduction reaction by the fungal cell wall is often known as argentaffin reaction.[2]
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Silver impregnation using methenamine silver
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Pneumocystis microscopy
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Methenamine silver borate stained smear of Candida albicans under microscope
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References
- ^ Nassar A, Zapata M, Little JV, Siddiqui MT (November 2006). "Utility of reflex gomori methenamine silver staining for Pneumocystis jirovecii on bronchoalveolar lavage cytologic specimens: A review". Diagn. Cytopathol. 34 (11): 719–23. doi:10.1002/dc.20540. PMID 17041954.
- ^ "Grocott-Gomori's Methenamine Silver Staining". Microbe Notes. 2021-02-08. Retrieved 2022-01-03.
This page was last edited on 2 June 2023, at 16:51