To install click the Add extension button. That's it.

The source code for the WIKI 2 extension is being checked by specialists of the Mozilla Foundation, Google, and Apple. You could also do it yourself at any point in time.

4,5
Kelly Slayton
Congratulations on this excellent venture… what a great idea!
Alexander Grigorievskiy
I use WIKI 2 every day and almost forgot how the original Wikipedia looks like.
Live Statistics
English Articles
Improved in 24 Hours
Added in 24 Hours
What we do. Every page goes through several hundred of perfecting techniques; in live mode. Quite the same Wikipedia. Just better.
.
Leo
Newton
Brights
Milds

From Wikipedia, the free encyclopedia

Neutral red
Neutral red
Solid Neutral Red
Neutral Red Aqueous Solution
Names
IUPAC name
N2,N2,7-Trimethylphenazine-2,8-diamine—hydrogen chloride (1/1)
Other names
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride
Toluylene red
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.008.215 Edit this at Wikidata
EC Number
  • 209-035-8
UNII
  • InChI=1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H checkY
    Key: PGSADBUBUOPOJS-UHFFFAOYSA-N checkY
  • InChI=1/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
    Key: PGSADBUBUOPOJS-UHFFFAOYAB
  • Cl.n1c3c(nc2c1cc(c(c2)N)C)cc(N(C)C)cc3
Properties
C15H17N4
Molar mass 288.78 g/mol
Melting point 290 °C (554 °F; 563 K)
Hazards
GHS labelling:
GHS06: Toxic
GHS07: Exclamation mark
GHS08: Health hazard
Danger
H301, H315, H319, H335, H341
P201, P202, P261, P264, P270, P271, P280, P281, P301+P310, P302+P352, P304+P340, P305+P351+P338, P308+P313, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)
Neutral red (pH indicator)
below pH 6.8 above pH 8.0
6.8 8.0

Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red.[1] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood. Can be used for staining Golgi apparatus in cells and Nissl granules in neurons.

In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation.

Neutral red can be used as a vital stain.[2] The Neutral Red Cytotoxicity Assay was first developed by Ellen Borenfreund in 1984. In the Neutral Red Assay live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability.[3] The neutral red is also used to stain cell cultures for plate titration of viruses.

Neutral red is added to some growth media for bacterial and cell cultures. It usually is available as a chloride salt.

Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0.

YouTube Encyclopedic

  • 1/3
    Views:
    9 328
    7 788
    2 016
  • Rojo Neutro. Indicador de pH en Zona Neutra.
  • reliable electric 12 volt inverter review hard wired black is neutral red is hot an yellow is ground
  • Lactose and non lactose fermenting bacteria | MacConkey agar (Clear explanation)

Transcription

References

  1. ^ Winckler, Jürgen (1973). Vitalfärbung von Lysosomen und anderen Zellorganellen der Ratte mit Neutralrot [Vital staining of lysosomes and other cell organelles of the rat with Neutral red]. Progress in Histochemistry and Cytochemistry (in German). Vol. 6 (3). Gustav Fischer Verlag. pp. 1–91. doi:10.1016/S0079-6336(74)80001-X. ISBN 3-437-10353-9. PMID 4142096.
  2. ^ Repetto, Guillermo; del Peso, Ana; Zurita, Jorge L. (2008). "Neutral red uptake assay for the estimation of cell viability/cytotoxicity". Nature Protocols. 3 (7): 1125–1131. doi:10.1038/nprot.2008.75. PMID 18600217. S2CID 24676983.
  3. ^ Borenfreund, Ellen; Puerner, James A. (1984). "A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR90)". Journal of Tissue Culture Methods. 9 (1): 7–9. doi:10.1007/BF01666038.

Other references

  • Borenfreund, Ellen; Puerner, James A. (1985). "Toxicity determined in vitro by morphological alterations and neutral red absorption". Toxicology Letters. 24 (2–3): 119–124. doi:10.1016/0378-4274(85)90046-3. PMID 3983963.
  • Borenfreund, E.; Babich, H.; Martin-Alguacil, N. (1988). "Comparisons of two in vitro cytotoxicity assays—The neutral red (NR) and tetrazolium MTT tests". Toxicology in Vitro. 2 (1): 1–6. doi:10.1016/0887-2333(88)90030-6. PMID 20702351.
This page was last edited on 5 May 2023, at 19:29
Basis of this page is in Wikipedia. Text is available under the CC BY-SA 3.0 Unported License. Non-text media are available under their specified licenses. Wikipedia® is a registered trademark of the Wikimedia Foundation, Inc. WIKI 2 is an independent company and has no affiliation with Wikimedia Foundation.