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Tinción negativa

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Cryptococcus neoformans revelados por medio de una tinción negativa con tinta china.

Tinción negativacontrastar las muestras mediante una sustancia opaca a los fotones (microscopía óptica) o a los electrones (microscopía electrónica). En el primer caso, se emplea nigrosina o tinta china; para el caso de bacteria, esta técnica permite visualizar las cápsulas como entes refringentes sobre un campo de fondo oscuro.[1]​ En caso de microscopía electrónica de transmisión, se emplean sustancias de alto número atómico que, por tanto, resultan opacas a los electrones transmitidos. Típicamente, estas sustancias son acetato de uranilo, citrato de plomo o molibdato de amonio. Al electrónico, esta técnica permite visualizar virus, flagelos, bacterias y otros entes de escaso tamaño.

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  • Tincion Negativa.
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  • Coloración de Gram y Ziehl Neelsen

Transcription

Referencias

  1. ↑ S. Woeste and P. Demchick (1991). Appl Environ Microbiol. 57(6): 1858-1859 http://aem.asm.org/cgi/content/abstract/57/6/1858
Esta página se editó por última vez el 9 may 2024 a las 07:43.
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