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From Wikipedia, the free encyclopedia

Widal test
Purposeserological test for enteric fever
Test ofTyphoid fever (enteric fever)
Based onSeropositivity: immune agglutination reaction to specific infectious agent

The Widal test, developed in 1896 and named after its inventor, Georges-Fernand Widal, is an indirect agglutination test for enteric fever or undulant fever whereby bacteria causing typhoid fever is mixed with a serum containing specific antibodies obtained from an infected individual. In cases of Salmonella infection, it is a demonstration of the presence of O-soma false-positive result. Test results need to be interpreted carefully to account for any history of enteric fever, typhoid vaccination, and the general level of antibodies in the populations in endemic areas of the world. As with all serological tests, the rise in antibody levels needed to perform the diagnosis takes 7–14 days, which limits its applicability in early diagnosis. Other means of diagnosing Salmonella typhi (and paratyphi) include cultures of blood, urine and faeces. These organisms produce H2S from thiosulfate and can be identified easily on differential media such as bismuth sulfite agar.[1][2][3] Typhidot is the other test used to ascertain the diagnosis of typhoid fever. A new serological test called the Tubex test is neither superior nor better performing than the Widal test. Therefore, Tubex test is not recommended for diagnosis of typhoid fever.[4]

2-mercaptoethanol is often added to the Widal test. This agent more easily denatures the IgM class of antibodies, so if a decrease in the titer is seen after using this agent, it means that the contribution of IgM has been removed leaving the IgG component. This differentiation of antibody classes is important as it allows for the distinction of a recent (IgM) from an old infection (IgG).

The Widal test is positive if TO antigen titer is more than 1:160 in an active infection, or if TH antigen titer is more than 1:160 in past infection or in immunized persons. A single Widal test is of little clinical relevance especially in endemic areas such as Indian subcontinent, Africa and South-east Asia. This is due to recurrent exposure to the typhoid causing bacteria, immunization and high chances of cross-reaction from infections, such as malaria and non typhoidal salmonella. [5]

If no other tests (either bacteriologic culture or more specific serology) are available, a fourfold increase in the titer (e.g., from 1:40 to 1:640) in the course of the infection, or a conversion from an IgM reaction to an IgG reaction of at least the same titer, would be consistent with a typhoid infection.

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Transcription

See also

References

  1. ^ Olopoenia, Lateef A.; King, Aprileona L. (1 February 2000). "Widal agglutination test − 100 years later: still plagued by controversy". Postgraduate Medical Journal. 76 (892): 80–84. doi:10.1136/pmj.76.892.80. ISSN 0032-5473. PMC 1741491. PMID 10644383.
  2. ^ Cheesbrough; Monica (2006). District Laboratory Practice in Tropical Countries (2nd ed.). Norfolk: Cambridge University Press. p. 185. ISBN 978-0-521-67631-1.
  3. ^ CDC Yellow Book 2020: Health Information for International Travel (2019). Gary W. Brunette (ed.). CDC Yellow Book 2020: Health Information for International Travel. Jeffrey B. nemhauser. New York: Oxford University Press. ISBN 978-0-19-092893-3.{{cite book}}: CS1 maint: numeric names: authors list (link)
  4. ^ Bakr WM, El Attar LA, Ashour MS, El Tokhy AM (2010). "TUBEX Test Versus Widal Test In The Diagnosis Of Typhoid Fever In Kafr El -Shekh, Egypt". J Egypt Public Health Assoc. 85 (5–6): 285–96. PMID 22054103.
  5. ^ "Widal Test". knowtreatment.com. 31 July 2020.

Further reading

This page was last edited on 29 February 2024, at 00:03
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