Ribosome-nascent chain complex

Ribosome-nascent chain complex (RNC) refers to the collection of molecules that constitute a ribosome attached to the polypeptide (protein) that it is synthesising. The synthesis of the nascent polypeptide may be stalled by one of several methods. RNCs are produced and purified in laboratories to study the dynamics, biochemistry, folding and interactions of both the ribosome and proteins undergoing synthesis.^[1]^[2]

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^ Cabrita LD, Dobson CM, Christodoulou J (February 2010). "Protein folding on the ribosome". Current Opinion in Structural Biology. 20 (1): 33–45. doi:10.1016/j.sbi.2010.01.005. PMID 20149635.
^ Schaffitzel C, Ban N (June 2007). "Generation of ribosome nascent chain complexes for structural and functional studies". Journal of Structural Biology. 158 (3): 463–71. doi:10.1016/j.jsb.2007.01.005. PMID 17350284.

v t e Structures of the cell / organelles
Endomembrane system	Cell membrane Nucleus Endoplasmic reticulum Golgi apparatus Parenthesome Autophagosome Vesicle Exosome Lysosome Endosome Phagosome Vacuole Acrosome Cytoplasmic granule Melanosome Microbody Glyoxysome Peroxisome Weibel–Palade body
Cytoskeleton	Microfilament Intermediate filament Microtubule Prokaryotic cytoskeleton Microtubule organizing center Centrosome Centriole Basal body Spindle pole body Myofibril Undulipodium Cilium Flagellum Axoneme Radial spoke Pseudopodium Lamellipodium Filopodium
Endosymbionts	Mitochondrion Plastid Chloroplast Chromoplast Gerontoplast Leucoplast Amyloplast Elaioplast Proteinoplast Tannosome
Other internal	Nucleolus RNA Ribosome Spliceosome Vault Cytoplasm Cytosol Inclusions Proteasome Magnetosome
External	Cell wall Extracellular matrix

Ribosomal RNA / ribosome subunits

Archaea
(70S)

Large (50S):

Small (30S):

Bacteria
(70S)

Large (50S):

Small (30S):

Eukaryotes

Cytoplasmic (80S)	Large (60S): 5S 5.8S 28S Small (40S): 18S
Mitochondrial (55S)	Large (28S): MT-RNR2, 16S MT-tRNA^Val Small (39S): MT-RNR1, 12S
Chloroplast (70S)	Large (50S): 5S 4.5S 23S Small (30S): 16S

Ribosomal proteins

(See article table)

Gene expression

Introduction
to genetics

Transcription

Types	Bacterial Archaeal Eukaryotic
Key elements	Transcription factor RNA polymerase Promoter
Post-transcription	Precursor mRNA (pre-mRNA / hnRNA) 5' capping Splicing Polyadenylation Histone acetylation and deacetylation

Translation

Types	Bacterial Archaeal Eukaryotic
Key elements	Ribosome Transfer RNA (tRNA) Ribosome-nascent chain complex (RNC) Post-translational modification

Regulation

Influential people

v t e Proteins: key methods of study
Experimental	Protein purification Green fluorescent protein Western blot Protein immunostaining Protein sequencing Gel electrophoresis/Protein electrophoresis Protein immunoprecipitation Peptide mass fingerprinting/Protein mass spectrometry Dual-polarization interferometry Microscale thermophoresis Chromatin immunoprecipitation Surface plasmon resonance Isothermal titration calorimetry X-ray crystallography Protein NMR Cryo-electron microscopy Freeze-fracture electron microscopy
Bioinformatics	Protein structure prediction Protein function prediction Protein–protein docking Protein structural alignment Protein ontology Protein–protein interaction prediction
Assay	Enzyme assay Protein assay Secretion assay
Display techniques	Bacterial display mRNA display Phage display Ribosome display Yeast display
Super-resolution microscopy	Photoactivated localization microscopy Vertico SMI

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This page was last edited on 26 October 2022, at 21:40

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