POLR2B

POLR2B

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
5IY9, 5IYA, 5IYC, 5IYB, 5IY7, 5IY8, 5IYD, 5IY6

Identifiers

Aliases

POLR2B, POL2RB, RPB2, hRPB140, hsRPB2, polymerase (RNA) II subunit B, RNA polymerase II subunit B

External IDs

OMIM: 180661 MGI: 2388280 HomoloGene: 722 GeneCards: POLR2B

Gene location (Human)

Chr.	Chromosome 4 (human)^[1]

Band	4q12	Start	56,977,722 bp^[1]
Band	4q12	End	57,031,158 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 5 (mouse)^[2]

Band	5\|5 C3.3	Start	77,457,994 bp^[2]
Band	5\|5 C3.3	End	77,497,171 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

germinal epithelium

endothelial cell

middle temporal gyrus

tibia

human penis

Brodmann area 23

parietal pleura

Achilles tendon

urethra

synovial joint

Top expressed in

saccule

otic placode

primitive streak

hair follicle

ciliary body

ureter

medullary collecting duct

retinal pigment epithelium

trigeminal ganglion

iris

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	transferase activity DNA binding nucleotidyltransferase activity ribonucleoside binding RNA polymerase II activity chromatin binding metal ion binding DNA-directed 5'-3' RNA polymerase activity protein binding RNA binding
Cellular component	membrane RNA polymerase II, core complex nucleus nucleoplasm
Biological process	mRNA splicing, via spliceosome somatic stem cell population maintenance transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription by RNA polymerase II positive regulation of viral transcription transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter snRNA transcription by RNA polymerase II fibroblast growth factor receptor signaling pathway transcription, DNA-templated RNA metabolic process regulation of gene silencing by miRNA
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


5431


231329

Ensembl


ENSG00000047315


ENSMUSG00000029250

UniProt


P30876


Q8CFI7

RefSeq (mRNA)


NM_001303269 NM_000938 NM_001303268


NM_153798

RefSeq (protein)


NP_000929 NP_001290197 NP_001290198


NP_722493

Location (UCSC)

Chr 4: 56.98 – 57.03 Mb

Chr 5: 77.46 – 77.5 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

DNA-directed RNA polymerase II subunit RPB2 is an enzyme that in humans is encoded by the POLR2B gene.^[5]^[6]

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.^[7]

Interactions

POLR2B has been shown to interact with POLR2C,^[8] POLR2E,^[8] POLR2H^[8] and POLR2L.^[8]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000047315 - Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000029250 - Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Acker J, Wintzerith M, Vigneron M, et al. (October 1992). "Primary structure of the second largest subunit of human RNA polymerase II (or B)". J Mol Biol. 226 (4): 1295–9. doi:10.1016/0022-2836(92)91071-V. PMID 1518060.
^ Acker J, Mattei MG, Wintzerith M, et al. (August 1994). "Chromosomal localization of human RNA polymerase II subunit genes". Genomics. 20 (3): 496–9. doi:10.1006/geno.1994.1208. PMID 8034326.
^ "Entrez Gene: POLR2B polymerase (RNA) II (DNA directed) polypeptide B, 140kDa".
^ ^a ^b ^c ^d Acker J, de Graaff M, Cheynel I, et al. (July 1997). "Interactions between the human RNA polymerase II subunits". J. Biol. Chem. 272 (27). UNITED STATES: 16815–21. doi:10.1074/jbc.272.27.16815. ISSN 0021-9258. PMID 9201987.

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Herrmann CH, Rice AP (1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". J. Virol. 69 (3): 1612–20. doi:10.1128/JVI.69.3.1612-1620.1995. PMC 188757. PMID 7853496.
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Yang X, Herrmann CH, Rice AP (1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". J. Virol. 70 (7): 4576–84. doi:10.1128/JVI.70.7.4576-4584.1996. PMC 190394. PMID 8676484.
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Interactions

References

Further reading