In ultrafiltration, the molecular weight cut-off or MWCO of a membrane refers to the lowest molecular weight of the solute (in daltons) for which 90% of the solute is retained by (prevented from passing through) the membrane,[1] or the molecular weight of the molecule (e.g. globular protein) that is 90% retained by the membrane.
YouTube Encyclopedic
-
1/3Views:208 404265 1683 096
-
Lab Protocol - Dialysis Tubing Experiments (Unit 7 Diffusion)
-
Trick To Find Molecular Mass / Molecular Weight | Find Molar Mass And Molar Weight In Just 2 Minutes
-
Protein Purification - Dialysis, Principle, Procedure and Factors affecting dialysis
Transcription
Details
This definition is not however standardized, and MWCOs can also be defined as the molecular weight at which 80% of the analytes (or solutes) are prohibited from membrane diffusion.
Commercially available microdialysis probes typically have molecular weight cutoffs that range from 1,000 to 300,000 Da, and larger thresholds of filtration are measured in μm. Microdialysis may also be used to separate nanoparticles from the solutions in which they were formed. In such a separation, the eluate will consist of non-complexed reactants and components.
Ultrafiltration membrane manufacturers commonly produce and offer MWCO's of 2k, 5k, 10k, 30k, 50k, 100k, and 1,000k. Devices offered range from laboratory focused centrifugal devices (100ul to 100ml) to laboratory and bioprocessing relevant tangential flow filtration (TFF) devices (50ml to hundreds of litres).
References
- ^ "Technical Resource Library from". Cole-Parmer. 2006-08-25. Retrieved 2013-06-20.
 | This biochemistry article is a stub. You can help Wikipedia by expanding it. |
This page was last edited on 16 May 2024, at 17:20