![Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]](http://upload.wikimedia.org/wikipedia/commons/f/f3/Summary_of_RNA-Seq.svg)
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Summary
| Description |
Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1] |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
| Other versions |
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- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:20, 13 February 2017 | | 1,020 × 803 (246 KB) | Evolution and evolvability | text as paths to avoid artefacts |
| 12:19, 13 February 2017 |  | 1,020 × 803 (90 KB) | Evolution and evolvability | text as paths to avoid artefacts | |
| 10:48, 13 February 2017 |  | 1,020 × 803 (88 KB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| 08:41, 10 February 2017 |  | 1,020 × 803 (87 KB) | Evolution and evolvability | reverse order of fragmentation and reverse-transcription | |
| 05:08, 5 October 2016 |  | 1,020 × 803 (85 KB) | Evolution and evolvability | User created page with UploadWizard |
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