![Cell populations in a high-dimensional mass-cytometry dataset manually gated after dimension reduction using 2D layout for a minimum spanning tree. Figure reproduced from the data provided in Note: reproduced from raw data published in: Sean C Bendall, Erin F Simonds, Peng Qiu, El-ad D Amir, Peter O Krutzik, Rachel Finck, Robert V Bruggner, Rachel Melamed, Angelica Trejo, Olga I Ornatsky, Robert S Balderas, Sylvia K Plevritis, Karen Sachs, Dana Pe'er, Scott D Tanner, Garry P Nolan (2011) Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332:687-96](http://upload.wikimedia.org/wikipedia/commons/thumb/9/90/Spade_plot.svg/420px-Spade_plot.svg.png)
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