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Summary
| Description |
Original figure legend: Pointlike emitter optical response. From left to right: calculated x-y (above) and r-z (below) intensity distributions, in logarithmic scale, for a point like source imaged by means of wide-field, 2PE and confocal microscopy. Both 2PE and confocal shapes exhibit a better signal to noise ratio than widefield case. 2PE distribution is larger due the fact that a wavelength twice than in the wide-field and confocal case is responsible for the intensity distribution. Such intensity distributions are also known as point spread functions of the related microscopes. Optical conditions: excitation wavelengths are 488 nm and 900 nm for 1PE and 2PE, respectively; emission wavelength is 520 nm; numerical aperture is 1.3 for an oil immersion objective with oil refractive index value set at 1.515.
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| Date | |
| Source |
Multi-photon excitation microscopy. BioMedical Engineering OnLine, 2006, 5:36. |
| Author | Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai |
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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| current | 18:57, 23 December 2008 | | 2,992 × 2,696 (1.93 MB) | Dietzel65 | == Beschreibung == {{Information |Description={{en|1=Original figure legend: ''Pointlike emitter optical response. From left to right: calculated x-y (above) and r-z (below) intensity distributions, in logarithmic scale, for a point like source imaged by |
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