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Summary
| Description |
Original figure legend: Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, Molecular Probes). Alexa Fluor 350 wheat germ agglutinin, a blue-fluorescent lectin, was used to stain the mucus of goblet cells. The filamentous actin prevalent in the brush border was stained with red-fluorescent Alexa Flu or 568 phalloidin. Finally, the nuclei were stained with SYTOX ® Green nucleic acid stain. Imaging has been performed at 780 nm, 100 x 1.4 NA Leica objective, using a Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) coupled at LAMBS-MicroScoBio with a Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS.
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| Date | Original version: 6 June 2006. Clipping: 4. March 2009. |
| Source |
Multi-photon excitation microscopy. BioMedical Engineering OnLine, 2006, 5:36. |
| Author |
Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai. |
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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| Other versions | For unclipped version see below |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 20:57, 4 March 2009 | | 714 × 467 (81 KB) | Dietzel65 | == Beschreibung == {{Information |Description={{en|1=Original figure legend: ''Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, |
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