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Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours at 37°C. Cells were processed for indirect immunofluorescence labelling using anti-lamin A/C antibodies (panel A, a to f), anti-lamin B1 antibodies (panel B, g to r) and anti-vimentin antibodies (panel B j to i and p to r). Control and HGPS fibroblasts are indicated. (A) Fibroblasts were immunostained with anti-lamin A/C antibodies. Note the increased number of dysmorphic nuclei in HGPS fibroblasts compared to control 24 hours after recovery from heat shock. (B) Fibroblasts were immunostained with anti-lamin B1 and vimentin at times indicated after heat shock. Bar, 10 μm. Paradisi et al. BMC Cell Biology 2005 6:27 doi:10.1186/1471-2121-6-27 |
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- 2010-01-05T15:52:16Z CopperKettle 900x1347 (279859 Bytes) {{Information |Description={{en|1=Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 16:08, 5 January 2010 | | 687 × 479 (66 KB) | CopperKettle | {{Information |Description={{en|1=Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours at 37°C. Cells were processed |
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