5-(carboxyamino)imidazole ribonucleotide mutase

In enzymology, a 5-(carboxyamino)imidazole ribonucleotide mutase (EC 5.4.99.18) is an enzyme that catalyzes the chemical reaction

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole ${\displaystyle \rightleftharpoons }$ 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

Hence, this enzyme has one substrate, 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole, and one product, 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole carboxymutase. Other names in common use include N5-CAIR mutase, PurE, N5-carboxyaminoimidazole ribonucleotide mutase, and class I PurE.

References

• Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM (1992). "Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway". Biochemistry. 31 (21): 5022–32. doi:10.1021/bi00136a016. PMID 1534690.
• Mueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J (1994). "N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli". Biochemistry. 33 (8): 2269–78. doi:10.1021/bi00174a038. PMID 8117684.
• Meyer E, Kappock TJ, Osuji C, Stubbe J (1999). "Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase". Biochemistry. 38 (10): 3012–8. doi:10.1021/bi9827159. PMID 10074353.
• Mathews II, Kappock TJ, Stubbe J, Ealick SE (1999). "Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway". Structure. 7 (11): 1395–406. doi:10.1016/S0969-2126(00)80029-5. PMID 10574791.
• Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ (1994). "Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms". Biochemistry. 33 (39): 11927–34. doi:10.1021/bi00205a031. PMID 7918411.
• Davisson VJ; Misialek, S; Toffaletti, DL; Klem, TJ; Perfect, JR; Davisson, VJ (1998). "Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis". Arch. Biochem. Biophys. 351 (1): 123–34. doi:10.1006/abbi.1997.0512. PMID 9500840.

This page was last edited on 26 August 2023, at 12:44