2-oxoglutarate synthase

In enzymology, a 2-oxoglutarate synthase (EC 1.2.7.3) is an enzyme that catalyzes the chemical reaction

2-oxoglutarate + CoA + 2 oxidized ferredoxin ${\displaystyle \rightleftharpoons }$ succinyl-CoA + CO₂ + 2 reduced ferredoxin

The 3 substrates of this enzyme are 2-oxoglutarate, CoA, and oxidized ferredoxin, whereas its 3 products are succinyl-CoA, CO₂, and reduced ferredoxin.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with an iron-sulfur protein as acceptor. The systematic name of this enzyme class is 2-oxoglutarate:ferredoxin oxidoreductase (decarboxylating). Other names in common use include 2-ketoglutarate ferredoxin oxidoreductase, 2-oxoglutarate:ferredoxin oxidoreductase, KGOR, 2-oxoglutarate ferredoxin oxidoreductase, and 2-oxoglutarate:ferredoxin 2-oxidoreductase (CoA-succinylating). This enzyme participates in the Citric acid cycle. Some forms catalyze the reverse reaction within the Reverse Krebs cycle, as a means of carbon fixation.

References

• Buchanan BB, Evans MC (1965). "The synthesis of alpha-ketoglutarate from succinate and carbon dioxide by a subcellular preparation of a photosynthetic bacterium". Proc. Natl. Acad. Sci. U.S.A. 54 (4): 1212–8. Bibcode:1965PNAS...54.1212B. doi:10.1073/pnas.54.4.1212. PMC 219840. PMID 4286833.
• Gehring U, Arnon DI (1972). "Purification and properties of -ketoglutarate synthase from a photosynthetic bacterium". J. Biol. Chem. 247 (21): 6963–9. doi:10.1016/S0021-9258(19)44680-2. PMID 4628267.
• Dorner E, Boll M (2002). "Properties of 2-Oxoglutarate:Ferredoxin Oxidoreductase from Thauera aromatica and Its Role in Enzymatic Reduction of the Aromatic Ring". J. Bacteriol. 184 (14): 3975–83. doi:10.1128/JB.184.14.3975-3983.2002. PMC 135165. PMID 12081970.
• Mai X, Adams MW (1996). "Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis". J. Bacteriol. 178 (20): 5890–6. doi:10.1128/jb.178.20.5890-5896.1996. PMC 178443. PMID 8830683.
• Schut GJ, Menon AL, Adams MW (2001). "2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis". Methods Enzymol. Methods in Enzymology. 331: 144–58. doi:10.1016/S0076-6879(01)31053-4. ISBN 978-0-12-182232-3. PMID 11265457.

This page was last edited on 26 August 2023, at 12:30