FLOT2

FLOT2

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1WIN

Identifiers

Aliases

FLOT2, ECS-1, ECS1, ESA, ESA1, M17S1, flotillin 2

External IDs

OMIM: 131560 MGI: 103309 HomoloGene: 3293 GeneCards: FLOT2

Gene location (Human)

Chr.	Chromosome 17 (human)^[1]

Band	17q11.2	Start	28,879,335 bp^[1]
Band	17q11.2	End	28,897,733 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 11 (mouse)^[2]

Band	11 B5\|11 46.74 cM	Start	77,928,757 bp^[2]
Band	11 B5\|11 46.74 cM	End	77,951,260 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

monocyte

spleen

blood

gastric mucosa

lymph node

minor salivary glands

right lung

popliteal artery

upper lobe of left lung

left coronary artery

Top expressed in

ankle joint

external carotid artery

facial motor nucleus

internal carotid artery

dorsomedial hypothalamic nucleus

motor neuron

carotid body

barrel cortex

atrium

ascending aorta

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protease binding protein binding protein heterodimerization activity ionotropic glutamate receptor binding
Cellular component	vesicle endosome membrane cell-cell contact zone focal adhesion endocytic vesicle acrosomal membrane flotillin complex uropod basolateral plasma membrane apical plasma membrane cortical actin cytoskeleton perinuclear region of cytoplasm caveola membrane raft extracellular exosome cytoplasmic vesicle lamellipodium plasma membrane dendrite cytoplasm synapse glutamatergic synapse anchored component of presynaptic active zone membrane
Biological process	positive regulation of establishment of T cell polarity regulation of toll-like receptor 3 signaling pathway regulation of heterotypic cell-cell adhesion protein stabilization negative regulation of amyloid precursor protein catabolic process negative regulation of gene expression protein localization to plasma membrane raft cell adhesion membrane raft assembly regulation of myoblast differentiation epidermis development protein localization to plasma membrane anterograde dendritic transport regulation of postsynaptic membrane neurotransmitter receptor levels positive regulation of NF-kappaB transcription factor activity
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


2319


14252

Ensembl


ENSG00000132589


ENSMUSG00000061981

UniProt


Q14254


Q60634

RefSeq (mRNA)


NM_004475 NM_001330170

NM_001040403 NM_001284227 NM_001284228 NM_008028 NM_001362625
NM_001362626 NM_001362627

RefSeq (protein)


NP_001317099 NP_004466

NP_001035493 NP_001271156 NP_001271157 NP_032054 NP_001349554
NP_001349555 NP_001349556

Location (UCSC)

Chr 17: 28.88 – 28.9 Mb

Chr 11: 77.93 – 77.95 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Flotillin-2 is a protein that in humans is encoded by the FLOT2 gene.^[5]^[6] Flotillin 2 (flot-2) is a highly conserved protein isolated from caveolae/lipid raft domains that tether growth factor receptors linked to signal transduction pathways. Flot-2 binds to PAR-1, a known upstream mediator of major signal transduction pathways implicated in cell growth and metastasis, and may influence tumour progression.^[7]

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.^[6]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000132589 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000061981 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Schroeder WT, Siciliano MJ, Stewart-Galetka SL, Duvic M (Feb 1992). "The human gene for an epidermal surface antigen (M17S1) is located at 17q11-12". Genomics. 11 (2): 481–2. doi:10.1016/0888-7543(91)90166-C. PMID 1769667.
^ ^a ^b "Entrez Gene: FLOT2 flotillin 2".
^ Hazarika P, McCarty MF, Prieto VG, George S, Babu D, Koul D, Bar-Eli M, Duvic M (Oct 2004). "Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1". Cancer Res. 64 (20): 7361–9. doi:10.1158/0008-5472.CAN-04-0823. PMID 15492257.

Schroeder WT, Stewart-Galetka S, Mandavilli S, et al. (1994). "Cloning and characterization of a novel epidermal cell surface antigen (ESA)". J. Biol. Chem. 269 (31): 19983–91. doi:10.1016/S0021-9258(17)32117-8. PMID 8051082.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Volonte D, Galbiati F, Li S, et al. (1999). "Flotillins/cavatellins are differentially expressed in cells and tissues and form a hetero-oligomeric complex with caveolins in vivo. Characterization and epitope-mapping of a novel flotillin-1 monoclonal antibody probe". J. Biol. Chem. 274 (18): 12702–9. doi:10.1074/jbc.274.18.12702. PMID 10212252.
Hazarika P, Dham N, Patel P, et al. (1999). "Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation". J. Cell. Biochem. 75 (1): 147–59. doi:10.1002/(SICI)1097-4644(19991001)75:1<147::AID-JCB15>3.0.CO;2-D. PMID 10462713. S2CID 12223151.
Salzer U, Prohaska R (2001). "Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts". Blood. 97 (4): 1141–3. doi:10.1182/blood.V97.4.1141. PMID 11159550.
Solomon S, Masilamani M, Rajendran L, et al. (2002). "The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs". Immunobiology. 205 (1): 108–19. doi:10.1078/0171-2985-00114. PMID 11999340.
Wistow G, Bernstein SL, Wyatt MK, et al. (2002). "Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants". Mol. Vis. 8: 205–20. PMID 12107410.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Shin BK, Wang H, Yim AM, et al. (2003). "Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function". J. Biol. Chem. 278 (9): 7607–16. doi:10.1074/jbc.M210455200. PMID 12493773.
Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Hazarika P, McCarty MF, Prieto VG, et al. (2004). "Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1". Cancer Res. 64 (20): 7361–9. doi:10.1158/0008-5472.CAN-04-0823. PMID 15492257.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Bracho C, Dunia I, Romano M, et al. (2006). "Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax". Parasitol. Res. 99 (2): 153–9. doi:10.1007/s00436-006-0139-6. PMID 16521037. S2CID 1099026.
Doherty SD, Prieto VG, George S, et al. (2007). "High flotillin-2 expression is associated with lymph node metastasis and Breslow depth in melanoma". Melanoma Res. 16 (5): 461–3. doi:10.1097/01.cmr.0000222592.75858.20. PMID 17013097. S2CID 9758718.
Jiang M, Ding Y, Su Y, et al. (2007). "Arginase-flotillin interaction brings arginase to red blood cell membrane". FEBS Lett. 580 (28–29): 6561–4. doi:10.1016/j.febslet.2006.11.003. PMID 17113085. S2CID 20180459.
Sugawara Y, Nishii H, Takahashi T, et al. (2007). "The lipid raft proteins flotillins/reggies interact with Galphaq and are involved in Gq-mediated p38 mitogen-activated protein kinase activation through tyrosine kinase". Cell. Signal. 19 (6): 1301–8. doi:10.1016/j.cellsig.2007.01.012. PMID 17307333.
Langhorst MF, Solis GP, Hannbeck S, et al. (2007). "Linking membrane microdomains to the cytoskeleton: regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin". FEBS Lett. 581 (24): 4697–703. doi:10.1016/j.febslet.2007.08.074. PMID 17854803. S2CID 13258838.
Rajendran L, Beckmann J, Magenau A, et al. (2009). "Flotillins are involved in the polarization of primitive and mature hematopoietic cells". PLOS ONE. 4 (12): e8290. Bibcode:2009PLoSO...4.8290R. doi:10.1371/journal.pone.0008290. PMC 2794375. PMID 20027317.
Pust S, Dyve AB, Torgersen ML, et al. (2010). "Interplay between toxin transport and flotillin localization". PLOS ONE. 5 (1): e8844. Bibcode:2010PLoSO...5.8844P. doi:10.1371/journal.pone.0008844. PMC 2809741. PMID 20107503.
Stuermer CA (2010). "The reggie/flotillin connection to growth". Trends Cell Biol. 20 (1): 6–13. doi:10.1016/j.tcb.2009.10.003. PMID 19896850.
Solis GP, Malaga-Trillo E, Plattner H, et al. (2010). "Cellular roles of the prion protein in association with reggie/flotillin microdomains" (PDF). Front. Biosci. 15 (3): 1075–85. doi:10.2741/3662. PMID 20515742.
Ludwig A, Otto GP, Riento K, et al. (2010). "Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment". J. Cell Biol. 191 (4): 771–84. doi:10.1083/jcb.201005140. PMC 2983060. PMID 21059848.
Cornfine S, Himmel M, Kopp P, et al. (2011). "The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes". Mol Biol Cell. 22 (2): 202–15. doi:10.1091/mbc.E10-05-0394. PMC 3020916. PMID 21119006.
Ge L, Qi W, Wang LJ, Miao HH, et al. (2011). "Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake". Proc Natl Acad Sci U S A. 108 (2): 551–6. Bibcode:2011PNAS..108..551G. doi:10.1073/pnas.1014434108. PMC 3021008. PMID 21187433.
Song J, Chen W, Lu Z, et al. (2011). "Soluble expression, purification, and characterization of recombinant human flotillin-2 (reggie-1) in Escherichia coli". Mol Biol Rep. 38 (3): 2091–8. doi:10.1007/s11033-010-0335-4. PMID 20857209. S2CID 26736046.

v t e PDB gallery
1win: Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein

This page was last edited on 13 October 2022, at 20:34

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References

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