 Chemical structure of fuchsin
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| Names | |
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| IUPAC name
4-[(E)-(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline;hydrochloride
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| Other names
Carbol-Fuchsin
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| Identifiers | |
3D model (JSmol)
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| ChEBI | |
| ECHA InfoCard | 100.021.897 |
| EC Number |
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PubChem CID
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CompTox Dashboard (EPA)
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| Properties | |
| C26H25N3O·HCl | |
| Molar mass | 351.9 g/mol |
| Hazards | |
| GHS labelling:[1] | |
 
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| Warning | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS ) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes.
It is a component of Ziehl–Neelsen stain, a differential stain.[2][3] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH).[4] Additionally, it can be used for the staining of bacterial spores.
Carbol-fuchsin is also used as a topical antiseptic and antifungal.[citation needed]
YouTube Encyclopedic
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1/3Views:12 696227 01889 979
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Preparing an Acid-Fast Stain using the Ziehl-Nielsen Method - Multi-Lingual Captions
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Acid-Fast Stain
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Ziehl Neelsen Stain (Z-N Stain) || Acid Fast Staining || ZN Stain ||Microbiology || By Manisha Ma'am
Transcription
(English captions by Andrea Matsumoto, University of Michigan.) Prepare sputum smear by taking the sputum from the sputum container using the loop, which has been sterilized. Spread it on the microscope slide very wide, about two to three centimeter diameter and then flame the loop again. Flame the sputum smear by fixing it. These are the spirit lamp and then the stains required for the staining procedure. Cover the smear with Carbol Fuchsin. And heat it with the spirit lamp intermittently until it steams but should not boil or the steam should not get dried and should be done intermittently for five minutes. Tip the steam off and allow it to cool before washing the excess stain from the slide of the smear. Cover the smear with the decolorizer is twenty percent sulfuric acid for two minutes. After two minutes tip off the decolorizer and wash it under the tap water or distilled water. Cover the smear with the counterstain with methylene blue for two minutes. No heating. Wash it under the tap water or distilled water. And air dry it on the draining rack. Put oil immersion on the smeared slide and then examine it under the times hundred (100X) objective of the microscope. And you are expected to see reddish or pinkish bacilli, which can appear in a form of a cocci or it can also appear as a spiral or it can appear as a dot. Subtitles by the Amara.org community
References
- ^ PubChem. "Carbol-Fuchsin". pubchem.ncbi.nlm.nih.gov. Retrieved 2023-07-29.
- ^ Angra P, Ridderhof J, Smithwick R (July 2003). "Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli". J. Clin. Microbiol. 41 (7): 3459. doi:10.1128/JCM.41.7.3459.2003. PMC 165351. PMID 12843125.
- ^ Selvakumar N, Rahman F, Rajasekaran S, et al. (August 2002). "Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli". J. Clin. Microbiol. 40 (8): 3041–3. doi:10.1128/JCM.40.8.3041-3043.2002. PMC 120628. PMID 12149374.
- ^ Sokolovská, Ivana; Rozenberg, Raoul; Riez, Christophe; et al. (2003-12-01). "Carbon Source-Induced Modifications in the Mycolic Acid Content and Cell Wall Permeability of Rhodococcus erythropolis E1". Applied and Environmental Microbiology. 69 (12): 7019–7027. Bibcode:2003ApEnM..69.7019S. doi:10.1128/AEM.69.12.7019-7027.2003. ISSN 0099-2240. PMC 309960. PMID 14660344.
This page was last edited on 22 September 2023, at 13:33